www.ctrt.net > ECoRI和 HinDIII具体是什么酶,rEstriCtion sitEs ...

ECoRI和 HinDIII具体是什么酶,rEstriCtion sitEs ...

限制性内切酶,能够特异性切割特定的回文序列 restriction sites HindIII and EcoRI就是指HindIII和EcoRI的限制性酶切位点

HindIII切割位点是:A|AGCTT EcoRI切割为点是:G|AATTC http://www.neb.com/nebecomm/products/productR0104.asp http://www.neb.com/nebecomm/products/productr0101.asp

Enzyme Cat # Temp Supplied NEBuffer SAM % Activity in NEBuffer 1.1 2.1 3.1 CutSmart EcoRI R0101 37°C NEBuffer EcoRI no 25 100* 50 50* HindIII R0104 37°C NEBuffer 2.1 no 25 100 50 50 Double Digest Recommendations for EcoRI + Hin...

看说明书~不同品牌不同系列酶切反应体系时间都不一样~

如图

DNA电泳一定要使用DNA Marker或已知大小的正对照DNA...如果选择λDNA/HindIII或者λDNA/EcoRI的酶切Marker...

补充回答: 看了楼主的问题补充,据此给一个补充回答:建议以现有TA克隆的质粒为模板,用两端分别带有EoRI和HindIII酶切位点的目的基因特异性引物再次PCR扩增..

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