www.ctrt.net > ECoRI和 HinDIII具体是什么酶,rEstriCtion sitEs ...

ECoRI和 HinDIII具体是什么酶,rEstriCtion sitEs ...

限制性内切酶,能够特异性切割特定的回文序列 restriction sites HindIII and EcoRI就是指HindIII和EcoRI的限制性酶切位点

HindIII切割位点是:A|AGCTT EcoRI切割为点是:G|AATTC http://www.neb.com/nebecomm/products/productR0104.asp http://www.neb.com/nebecomm/products/productr0101.asp

Enzyme Cat # Temp Supplied NEBuffer SAM % Activity in NEBuffer 1.1 2.1 3.1 CutSmart EcoRI R0101 37°C NEBuffer EcoRI no 25 100* 50 50* HindIII R0104 37°C NEBuffer 2.1 no 25 100 50 50 Double Digest Recommendations for EcoRI + Hin...

你只用一种酶切 切出来的粘性末端是可以匹配的 所以会自身环化

看说明书~不同品牌不同系列酶切反应体系时间都不一样~

我发现你是什么都不会埃。。。

The extra nucleotides from the HindIII site ...Further, XbaI, EcoRI, and XhoI sites at the ...to generate a set of new restriction sites, XbaI...

这是缩写,怎么读你应该问你的中学生物老师,我也不知道,忘了。可能也许从没读对过

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